ELISA KITS
vWF Activity Kit
The von Willebrand Factor Activity Test is an ELISA for the quantitative detection of vWF activity in citrated human plasma. The assay utilizes a purified murine anti-vWF monoclonal antibody which recognizes a functional epitope on the vWF molecule to assess vWF activity levels. Results are reported in percent (%) of normal, relative to a calibrator that has been standardized against the third International Standard for Factor VIII and von WIllebrand Factor in plasma.
Each ABP von Willebrand Factor (vWF) Activity 96-microwell Test Kit contains the following:
- 12 x 8 stabilized antibody coated microwells (12 strips of 8 breakaway wells), with frame. Wells are coated with a monoclonal antibody specific for the functional region of vWF.
- 60mL Sample Diluent
- 3 x 0.5mL lyophilized Reference Plasma for preparation of reference curve, with assay sheet.
- 12mL HRP conjugated anti-human vWF Antibody Solution
- 13mL Substrate (TMB/H2O2)
- 15mL Stopping Solution (0.36N sulfuric acid)
- 30mL Wash Concentrate [33X phosphate buffered saline (PBS) with 0.01% Tween 20]
vWF Total Antigen Kit
The von Willebrand Factor Antigen Test Kit is an enzyme linked immunosorbent assay for determining von Willebrand Factor levels in human plasma, expressed in relative percent (%) of normal. The assay is intended to be used as an aid in the diagnosis of von Willebrand Disease in patients with bleeding disorders, and to help distinguish between vWD and classic Hemophilia A. The von Willebrand Factor Antigen Test Kit will accurately detect antigen levels as low as 5% of normal.
Each ABP von Willebrand Factor (vWF) Antigen 96-microwell Test Kit contains the following:
- 12 x 8 anti-human von Willebrand Factor antibody coated microwells (12 strips of 8 breakaway wells)
- 60mL Sample Diluent
- 3 x 0.5mL lyophilized Reference Plasma for preparation of reference curve, with assay sheet.
- 12mL HRP conjugated anti-human vWF Antibody Solution
- 13mL Substrate (TMB/H2O2)
- 15mL Stopping Solution (0.36N sulfuric acid)
- 30mL Wash Concentrate [33X phosphate buffered saline (PBS) with 0.01% Tween 20]